BD HorizonTM R718 Mouse Anti-Mouse SLAMF9

Description

Multicolor flow cytometric analysis of SLAMF9 expression by Mouse splenic plasmacytoid dendritic cells. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD19 Cat. No. 553785 , BUV395 Rat Anti-Mouse CD45R/B220 Cat. No. 563793 , BD Horizon BV421 Rat Anti-Mouse CD317 BST2 Cat. No. 566431 antibodies, and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse SLAMF9 antibody Cat. No. 569819/569894; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of SLAMF9 or Ig Isotype control staining versus CD317 BST2 was derived by progressive gating on B220/CD45R+ Lineage-negative cells CD3e-CD19- with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of SLAMF9 expression by Mouse splenic plasmacytoid dendritic cells. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD19 Cat. No. 553785 , BUV395 Rat Anti-Mouse CD45R/B220 Cat. No. 563793 , BD Horizon BV421 Rat Anti-Mouse CD317 BST2 Cat. No. 566431 antibodies, and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse SLAMF9 antibody Cat. No. 569819/569894; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of SLAMF9 or Ig Isotype control staining versus CD317 BST2 was derived by progressive gating on B220/CD45R+ Lineage-negative cells CD3e-CD19- with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of SLAMF9 expression by Mouse splenic plasmacytoid dendritic cells. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD19 Cat. No. 553785 , BUV395 Rat Anti-Mouse CD45R/B220 Cat. No. 563793 , BD Horizon BV421 Rat Anti-Mouse CD317 BST2 Cat. No. 566431 antibodies, and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse SLAMF9 antibody Cat. No. 569819/569894; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of SLAMF9 or Ig Isotype control staining versus CD317 BST2 was derived by progressive gating on B220/CD45R+ Lineage-negative cells CD3e-CD19- with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of SLAMF9 expression by Mouse splenic plasmacytoid dendritic cells. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD19 Cat. No. 553785 , BUV395 Rat Anti-Mouse CD45R/B220 Cat. No. 563793 , BD Horizon BV421 Rat Anti-Mouse CD317 BST2 Cat. No. 566431 antibodies, and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse SLAMF9 antibody Cat. No. 569819/569894; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of SLAMF9 or Ig Isotype control staining versus CD317 BST2 was derived by progressive gating on B220/CD45R+ Lineage-negative cells CD3e-CD19- with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of SLAMF9 expression by Mouse splenic plasmacytoid dendritic cells. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD19 Cat. No. 553785 , BUV395 Rat Anti-Mouse CD45R/B220 Cat. No. 563793 , BD Horizon BV421 Rat Anti-Mouse CD317 BST2 Cat. No. 566431 antibodies, and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse SLAMF9 antibody Cat. No. 569819/569894; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of SLAMF9 or Ig Isotype control staining versus CD317 BST2 was derived by progressive gating on B220/CD45R+ Lineage-negative cells CD3e-CD19- with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of SLAMF9 expression by Mouse splenic plasmacytoid dendritic cells. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD19 Cat. No. 553785 , BUV395 Rat Anti-Mouse CD45R/B220 Cat. No. 563793 , BD Horizon BV421 Rat Anti-Mouse CD317 BST2 Cat. No. 566431 antibodies, and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse SLAMF9 antibody Cat. No. 569819/569894; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of SLAMF9 or Ig Isotype control staining versus CD317 BST2 was derived by progressive gating on B220/CD45R+ Lineage-negative cells CD3e-CD19- with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.