BD HorizonTM R718 Mouse Anti-MR1

Description

Flow cytometric analysis of MR1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histograms or BD Horizon R718 Mouse anti-MR1 antibody Cat. No. 568420/568421; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on viable B16-F0 cells. Cells from the Mouse B16-F0 Skin Melanoma, ATCC CRL-6322 cell line were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; Right Plot as indicated. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histograms or BD Horizon R718 Mouse Anti-MR1 antibody Cat. No. 568420/568421; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histograms or BD Horizon R718 Mouse anti-MR1 antibody Cat. No. 568420/568421; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of MR1 expression on viable B16-F0 cells. Cells from the Mouse B16-F0 Skin Melanoma, ATCC CRL-6322 cell line were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; Right Plot as indicated. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histograms or BD Horizon R718 Mouse Anti-MR1 antibody Cat. No. 568420/568421; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of MR1 expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histograms or BD Horizon R718 Mouse anti-MR1 antibody Cat. No. 568420/568421; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on viable B16-F0 cells. Cells from the Mouse B16-F0 Skin Melanoma, ATCC CRL-6322 cell line were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; Right Plot as indicated. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histograms or BD Horizon R718 Mouse Anti-MR1 antibody Cat. No. 568420/568421; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.