BD HorizonTM R718 Mouse Anti-TCF-7/TCF-1

Description

Multicolor flow cytometric analysis of TCF-7/TCF-1 expression by Human lymphocytes or Mouse splenocytes. Bivariate pseudocolor density plots showing TCF-7/TCF-1 expression or Ig Isotype control staining versus CD4 were derived from gated events with the side and forward light-scattering characteristics of intact human lymphocytes upper plots or mouse splenocytes lower plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper Plots: Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561844/561843 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 567587; Right Plot at 0.5 mug/test. Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 740208 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon R718 Mouse Anti-TCF-7/TCF-1 antibody Right Plot at 0.5 mug/test. Multicolor flow cytometric analysis of TCF-7/TCF-1 expression by Human lymphocytes or Mouse splenocytes. Bivariate pseudocolor density plots showing TCF-7/TCF-1 expression or Ig Isotype control staining versus CD4 were derived from gated events with the side and forward light-scattering characteristics of intact human lymphocytes upper plots or mouse splenocytes lower plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper Plots: Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561844/561843 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 567587; Right Plot at 0.5 mug/test. Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 740208 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon R718 Mouse Anti-TCF-7/TCF-1 antibody Right Plot at 0.5 mug/test. Show More Multicolor flow cytometric analysis of TCF-7/TCF-1 expression by Human lymphocytes or Mouse splenocytes. Bivariate pseudocolor density plots showing TCF-7/TCF-1 expression or Ig Isotype control staining versus CD4 were derived from gated events with the side and forward light-scattering characteristics of intact human lymphocytes upper plots or mouse splenocytes lower plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper Plots: Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561844/561843 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 567587; Right Plot at 0.5 mug/test. Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 740208 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon R718 Mouse Anti-TCF-7/TCF-1 antibody Right Plot at 0.5 mug/test.