BD HorizonTM R718 Rat Anti-Blimp-1

Description

Multicolor flow cytometric analysis of Blimp-1 expression in Human Peripheral Blood Lymphocytes. Human peripheral blood mononuclear cells PBMC were stained in BD Horizon Brilliant Stain Buffer Cat. No. 566349 with BD Horizon BUV737 Mouse Anti-Human CD27 Cat No. 612829 and BD Horizon BUV395 Mouse Anti-Human CD19 Cat. No. 563549 antibodies and washed. The cells were then fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with BD Horizon R718 Rat Anti-Blimp-1 antibody Cat. No. 567764 at 1 mug/test. The bivariate pseudocolor density plot Left Panel showing the correlated expression of CD19 versus CD27 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Histograms Right Panel showing Blimp-1 expression for the three B cell subsets defined by the correlated expression of CD19 versus CD27 were derived from gated events as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Blimp-1 expression in Human Peripheral Blood Lymphocytes. Human peripheral blood mononuclear cells PBMC were stained in BD Horizon Brilliant Stain Buffer Cat. No. 566349 with BD Horizon BUV737 Mouse Anti-Human CD27 Cat No. 612829 and BD Horizon BUV395 Mouse Anti-Human CD19 Cat. No. 563549 antibodies and washed. The cells were then fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with BD Horizon R718 Rat Anti-Blimp-1 antibody Cat. No. 567764 at 1 mug/test. The bivariate pseudocolor density plot Left Panel showing the correlated expression of CD19 versus CD27 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Histograms Right Panel showing Blimp-1 expression for the three B cell subsets defined by the correlated expression of CD19 versus CD27 were derived from gated events as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Blimp-1 expression in Human Peripheral Blood Lymphocytes. Human peripheral blood mononuclear cells PBMC were stained in BD Horizon Brilliant Stain Buffer Cat. No. 566349 with BD Horizon BUV737 Mouse Anti-Human CD27 Cat No. 612829 and BD Horizon BUV395 Mouse Anti-Human CD19 Cat. No. 563549 antibodies and washed. The cells were then fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with BD Horizon R718 Rat Anti-Blimp-1 antibody Cat. No. 567764 at 1 mug/test. The bivariate pseudocolor density plot Left Panel showing the correlated expression of CD19 versus CD27 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Histograms Right Panel showing Blimp-1 expression for the three B cell subsets defined by the correlated expression of CD19 versus CD27 were derived from gated events as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Blimp-1 expression in Human Peripheral Blood Lymphocytes. Human peripheral blood mononuclear cells PBMC were stained in BD Horizon Brilliant Stain Buffer Cat. No. 566349 with BD Horizon BUV737 Mouse Anti-Human CD27 Cat No. 612829 and BD Horizon BUV395 Mouse Anti-Human CD19 Cat. No. 563549 antibodies and washed. The cells were then fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with BD Horizon R718 Rat Anti-Blimp-1 antibody Cat. No. 567764 at 1 mug/test. The bivariate pseudocolor density plot Left Panel showing the correlated expression of CD19 versus CD27 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Histograms Right Panel showing Blimp-1 expression for the three B cell subsets defined by the correlated expression of CD19 versus CD27 were derived from gated events as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Blimp-1 expression in Human Peripheral Blood Lymphocytes. Human peripheral blood mononuclear cells PBMC were stained in BD Horizon Brilliant Stain Buffer Cat. No. 566349 with BD Horizon BUV737 Mouse Anti-Human CD27 Cat No. 612829 and BD Horizon BUV395 Mouse Anti-Human CD19 Cat. No. 563549 antibodies and washed. The cells were then fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with BD Horizon R718 Rat Anti-Blimp-1 antibody Cat. No. 567764 at 1 mug/test. The bivariate pseudocolor density plot Left Panel showing the correlated expression of CD19 versus CD27 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Histograms Right Panel showing Blimp-1 expression for the three B cell subsets defined by the correlated expression of CD19 versus CD27 were derived from gated events as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Blimp-1 expression in Human Peripheral Blood Lymphocytes. Human peripheral blood mononuclear cells PBMC were stained in BD Horizon Brilliant Stain Buffer Cat. No. 566349 with BD Horizon BUV737 Mouse Anti-Human CD27 Cat No. 612829 and BD Horizon BUV395 Mouse Anti-Human CD19 Cat. No. 563549 antibodies and washed. The cells were then fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . Fixed cells were stained with BD Horizon R718 Rat Anti-Blimp-1 antibody Cat. No. 567764 at 1 mug/test. The bivariate pseudocolor density plot Left Panel showing the correlated expression of CD19 versus CD27 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Histograms Right Panel showing Blimp-1 expression for the three B cell subsets defined by the correlated expression of CD19 versus CD27 were derived from gated events as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.