BD HorizonTM R718 Rat Anti-Galectin-3

Description

Galectin-3 Expression by Human and Mouse Leucocytes 1. SurfaceNeutrophils Human blood was stained with BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; dashed histogram or BD Horizon R718 Rat Anti-Galectin-3 Cat. No. 568404/568405; solid line histogram at 1 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A histogram showing Surface Galectin-3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable neutrophils. 2. Surface + IntracellularPBMC Peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with PE Mouse Anti-Human CD11b Cat. No. 555388 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control or BD Horizon R718 Rat Anti-Galectin-3. The bivariate pseudocolor density plot shows the correlated expression of total cellular Galectin-3 or Ig Isotype control staining versus CD11b by gated intact PBMC. 3. SurfacePEC Thioglycolate-elicited C57BL/6 Mouse peritoneal exudate cells Thio-PEC were preincubated with Purified Rat Anti-Mouse CD16/CD32 Ab Mouse BD Fc Block and stained with either BD Horizon R718 Rat IgG2a, kappa Isotype Control dashed line histogram or BD Horizon R718 Rat Anti-Galectin-3 solid line histogram at 1 mug/test. The histogram shows Surface Galectin-3 expression or Ig Isotype control staining on viable DAPI-negative Cat. 564907 Thio-PEC. 4. Surface + IntracellularPEC Thio-PEC were similarly fixed, permeabilized and stained without DAPI . The histogram shows total cellular Galectin-3 expression or Ig Isotype control staining by intact Thio-PEC. Flow cytometry and analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown are not lot specific. Galectin-3 Expression by Human and Mouse Leucocytes 1. SurfaceNeutrophils Human blood was stained with BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; dashed histogram or BD Horizon R718 Rat Anti-Galectin-3 Cat. No. 568404/568405; solid line histogram at 1 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A histogram showing Surface Galectin-3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable neutrophils. 2. Surface + IntracellularPBMC Peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with PE Mouse Anti-Human CD11b Cat. No. 555388 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control or BD Horizon R718 Rat Anti-Galectin-3. The bivariate pseudocolor density plot shows the correlated expression of total cellular Galectin-3 or Ig Isotype control staining versus CD11b by gated intact PBMC. 3. SurfacePEC Thioglycolate-elicited C57BL/6 Mouse peritoneal exudate cells Thio-PEC were preincubated with Purified Rat Anti-Mouse CD16/CD32 Ab Mouse BD Fc Block and stained with either BD Horizon R718 Rat IgG2a, kappa Isotype Control dashed line histogram or BD Horizon R718 Rat Anti-Galectin-3 solid line histogram at 1 mug/test. The histogram shows Surface Galectin-3 expression or Ig Isotype control staining on viable DAPI-negative Cat. 564907 Thio-PEC. 4. Surface + IntracellularPEC Thio-PEC were similarly fixed, permeabilized and stained without DAPI . The histogram shows total cellular Galectin-3 expression or Ig Isotype control staining by intact Thio-PEC. Flow cytometry and analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown are not lot specific. Show More Galectin-3 Expression by Human and Mouse Leucocytes 1. SurfaceNeutrophils Human blood was stained with BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; dashed histogram or BD Horizon R718 Rat Anti-Galectin-3 Cat. No. 568404/568405; solid line histogram at 1 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A histogram showing Surface Galectin-3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable neutrophils. 2. Surface + IntracellularPBMC Peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with PE Mouse Anti-Human CD11b Cat. No. 555388 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control or BD Horizon R718 Rat Anti-Galectin-3. The bivariate pseudocolor density plot shows the correlated expression of total cellular Galectin-3 or Ig Isotype control staining versus CD11b by gated intact PBMC. 3. SurfacePEC Thioglycolate-elicited C57BL/6 Mouse peritoneal exudate cells Thio-PEC were preincubated with Purified Rat Anti-Mouse CD16/CD32 Ab Mouse BD Fc Block and stained with either BD Horizon R718 Rat IgG2a, kappa Isotype Control dashed line histogram or BD Horizon R718 Rat Anti-Galectin-3 solid line histogram at 1 mug/test. The histogram shows Surface Galectin-3 expression or Ig Isotype control staining on viable DAPI-negative Cat. 564907 Thio-PEC. 4. Surface + IntracellularPEC Thio-PEC were similarly fixed, permeabilized and stained without DAPI . The histogram shows total cellular Galectin-3 expression or Ig Isotype control staining by intact Thio-PEC. Flow cytometry and analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown are not lot specific.