BD HorizonTM R718 Rat Anti-Human IL-2

Description

Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941, Left Plot or BD Horizon R718 Rat Anti-Human IL-2 antibody Cat No. 567057/567248, Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. A bivariate pseudocolor density plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941, Left Plot or BD Horizon R718 Rat Anti-Human IL-2 antibody Cat No. 567057/567248, Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. A bivariate pseudocolor density plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941, Left Plot or BD Horizon R718 Rat Anti-Human IL-2 antibody Cat No. 567057/567248, Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. A bivariate pseudocolor density plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941, Left Plot or BD Horizon R718 Rat Anti-Human IL-2 antibody Cat No. 567057/567248, Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. A bivariate pseudocolor density plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941, Left Plot or BD Horizon R718 Rat Anti-Human IL-2 antibody Cat No. 567057/567248, Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. A bivariate pseudocolor density plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941, Left Plot or BD Horizon R718 Rat Anti-Human IL-2 antibody Cat No. 567057/567248, Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. A bivariate pseudocolor density plots showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.