BD HorizonTM R718 Rat Anti-Mouse CD117 (c-kit)

Description

Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567841; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567841; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567841; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567841; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567841; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD117 c-kit expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 563793 and with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Rat Anti-Mouse CD117 c-kit antibody Cat. No. 567841; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific.