BD HorizonTM R718 Rat Anti-Mouse CD200R3

Description

Multiparameter flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD49b antibody Cat. No. 553858/561066 and with either BD Horizon Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD200R3 antibody Cat. No. 567842; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD49b antibody Cat. No. 553858/561066 and with either BD Horizon Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD200R3 antibody Cat. No. 567842; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD49b antibody Cat. No. 553858/561066 and with either BD Horizon Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD200R3 antibody Cat. No. 567842; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD49b antibody Cat. No. 553858/561066 and with either BD Horizon Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD200R3 antibody Cat. No. 567842; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD49b antibody Cat. No. 553858/561066 and with either BD Horizon Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD200R3 antibody Cat. No. 567842; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD49b antibody Cat. No. 553858/561066 and with either BD Horizon Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD200R3 antibody Cat. No. 567842; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.