BD HorizonTM R718 Rat Anti-Mouse CD205 (DEC-205)

Description

Multiparameter flow cytometric analysis of CD205 DEC-205 expressed on mouse bone marrow cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 561084/553128 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567643; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD205 DEC-205 expressed on mouse bone marrow cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 561084/553128 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567643; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD205 DEC-205 expressed on mouse bone marrow cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 561084/553128 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567643; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD205 DEC-205 expressed on mouse bone marrow cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 561084/553128 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567643; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD205 DEC-205 expressed on mouse bone marrow cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 561084/553128 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567643; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD205 DEC-205 expressed on mouse bone marrow cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 561084/553128 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567643; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.