BD HorizonTM R718 Rat Anti-Mouse CD21/CD35 (CR2/CR1)

Description

Two- color flow cytometric analysis of CD21/CD35 CR2/CR1 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat No. 562922 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD21/CD35 CR2/CR1 antibody Cat No. 568633/568634; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 CR2/CR1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative mouse splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two- color flow cytometric analysis of CD21/CD35 CR2/CR1 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat No. 562922 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD21/CD35 CR2/CR1 antibody Cat No. 568633/568634; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 CR2/CR1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative mouse splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two- color flow cytometric analysis of CD21/CD35 CR2/CR1 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat No. 562922 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD21/CD35 CR2/CR1 antibody Cat No. 568633/568634; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 CR2/CR1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative mouse splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two- color flow cytometric analysis of CD21/CD35 CR2/CR1 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat No. 562922 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD21/CD35 CR2/CR1 antibody Cat No. 568633/568634; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 CR2/CR1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative mouse splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two- color flow cytometric analysis of CD21/CD35 CR2/CR1 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat No. 562922 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD21/CD35 CR2/CR1 antibody Cat No. 568633/568634; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 CR2/CR1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative mouse splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two- color flow cytometric analysis of CD21/CD35 CR2/CR1 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat No. 562922 and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD21/CD35 CR2/CR1 antibody Cat No. 568633/568634; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 CR2/CR1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative mouse splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.