BD HorizonTM R718 Rat Anti-Mouse CD25 (IL-2 Receptor alpha)

Description

Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730 and with either no BD Horizon R718 conjugated antibody Autofluorescence control; Left Plot or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570355/570425; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or cellular Autofluorescence versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Mouse splenic leukocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either no BD Horizon R718 conjugated antibody Autofluorescence control; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or cellular Autofluorescence was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730 and with either no BD Horizon R718 conjugated antibody Autofluorescence control; Left Plot or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570355/570425; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or cellular Autofluorescence versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Mouse splenic leukocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either no BD Horizon R718 conjugated antibody Autofluorescence control; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or cellular Autofluorescence was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730 and with either no BD Horizon R718 conjugated antibody Autofluorescence control; Left Plot or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570355/570425; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or cellular Autofluorescence versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Mouse splenic leukocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either no BD Horizon R718 conjugated antibody Autofluorescence control; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or cellular Autofluorescence was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730 and with either no BD Horizon R718 conjugated antibody Autofluorescence control; Left Plot or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570355/570425; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or cellular Autofluorescence versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Mouse splenic leukocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either no BD Horizon R718 conjugated antibody Autofluorescence control; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or cellular Autofluorescence was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730 and with either no BD Horizon R718 conjugated antibody Autofluorescence control; Left Plot or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570355/570425; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or cellular Autofluorescence versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Mouse splenic leukocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either no BD Horizon R718 conjugated antibody Autofluorescence control; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or cellular Autofluorescence was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730 and with either no BD Horizon R718 conjugated antibody Autofluorescence control; Left Plot or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570355/570425; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or cellular Autofluorescence versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Mouse splenic leukocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either no BD Horizon R718 conjugated antibody Autofluorescence control; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or cellular Autofluorescence was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.