BD HorizonTM R718 Rat Anti-Mouse CD4

Description

Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 Cat. No. 566939/ 566940; Right Plot at 0.125 mug/test. A two-parameter pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 Cat. No. 566939/ 566940; Right Plot at 0.125 mug/test. A two-parameter pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 Cat. No. 566939/ 566940; Right Plot at 0.125 mug/test. A two-parameter pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 Cat. No. 566939/ 566940; Right Plot at 0.125 mug/test. A two-parameter pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 Cat. No. 566939/ 566940; Right Plot at 0.125 mug/test. A two-parameter pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 Cat. No. 566939/ 566940; Right Plot at 0.125 mug/test. A two-parameter pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.