BD HorizonTM R718 Rat Anti-Mouse CD4

Description

Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 antibody Cat. No. 567312; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD4 expression or Ig Isotype staining versus CD3e were derived from gated events with the side and forward light-scattering characteristics of viable 7-AAD negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 antibody Cat. No. 567312; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD4 expression or Ig Isotype staining versus CD3e were derived from gated events with the side and forward light-scattering characteristics of viable 7-AAD negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 antibody Cat. No. 567312; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD4 expression or Ig Isotype staining versus CD3e were derived from gated events with the side and forward light-scattering characteristics of viable 7-AAD negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 antibody Cat. No. 567312; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD4 expression or Ig Isotype staining versus CD3e were derived from gated events with the side and forward light-scattering characteristics of viable 7-AAD negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 antibody Cat. No. 567312; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD4 expression or Ig Isotype staining versus CD3e were derived from gated events with the side and forward light-scattering characteristics of viable 7-AAD negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD4 antibody Cat. No. 567312; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD4 expression or Ig Isotype staining versus CD3e were derived from gated events with the side and forward light-scattering characteristics of viable 7-AAD negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.