BD HorizonTM R718 Rat Anti-Mouse CD40

Description

Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553032/553033/561095 antibodies and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD40 antibody Cat. No. 567846; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A two-color pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553032/553033/561095 antibodies and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD40 antibody Cat. No. 567846; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A two-color pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553032/553033/561095 antibodies and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD40 antibody Cat. No. 567846; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A two-color pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553032/553033/561095 antibodies and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD40 antibody Cat. No. 567846; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A two-color pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553032/553033/561095 antibodies and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD40 antibody Cat. No. 567846; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A two-color pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553032/553033/561095 antibodies and with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD40 antibody Cat. No. 567846; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A two-color pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.