BD HorizonTM R718 Rat Anti-Mouse CD73

Description

Two-color flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/c Bottom Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565/565992 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plots or BD Horizon R718 Rat Anti-Mouse CD73 antibody Cat. No. 567644; Right Plots at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI- lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/c Bottom Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565/565992 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plots or BD Horizon R718 Rat Anti-Mouse CD73 antibody Cat. No. 567644; Right Plots at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI- lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/c Bottom Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565/565992 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plots or BD Horizon R718 Rat Anti-Mouse CD73 antibody Cat. No. 567644; Right Plots at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI- lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.