BD HorizonTM R718 Rat Anti-Mouse CD8a

Description

Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/ and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8a Cat. No. 566985; Right Plot at 0.5 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/ and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8a Cat. No. 566985; Right Plot at 0.5 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/ and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8a Cat. No. 566985; Right Plot at 0.5 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/ and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8a Cat. No. 566985; Right Plot at 0.5 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/ and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8a Cat. No. 566985; Right Plot at 0.5 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/ and either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8a Cat. No. 566985; Right Plot at 0.5 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.