BD HorizonTM R718 Rat Anti-Mouse CD8b

Description

Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR mouse splenic leucocyte populations were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 , and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 567737; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD8b expression or Ig isotype control versus CD3e were derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR mouse splenic leucocyte populations were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 , and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 567737; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD8b expression or Ig isotype control versus CD3e were derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR mouse splenic leucocyte populations were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 , and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 567737; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD8b expression or Ig isotype control versus CD3e were derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR mouse splenic leucocyte populations were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 , and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 567737; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD8b expression or Ig isotype control versus CD3e were derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR mouse splenic leucocyte populations were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 , and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 567737; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD8b expression or Ig isotype control versus CD3e were derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR mouse splenic leucocyte populations were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 , and either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 567737; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing CD8b expression or Ig isotype control versus CD3e were derived from gated events with the side and forward light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.