BD HorizonTM R718 Rat Anti-Mouse CD8b

Description

Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 569480/569481; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 569480/569481; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 569480/569481; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 569480/569481; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 569480/569481; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either BD Horizon R718 Rat IgG1, kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse CD8b antibody Cat. No. 569480/569481; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.