
567302 ·
BDB Bioscience · Cat: 567302
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD90.2 expression on mouse thymocytes. Balb/c mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD90.2 antibody Cat. No. 567302; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histogram showing CD90.2 expression or Ig Isotype control staining was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD90.2 expression on mouse thymocytes. Balb/c mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD90.2 antibody Cat. No. 567302; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histogram showing CD90.2 expression or Ig Isotype control staining was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD90.2 expression on mouse thymocytes. Balb/c mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No. 566943; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD90.2 antibody Cat. No. 567302; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histogram showing CD90.2 expression or Ig Isotype control staining was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.