BD HorizonTM R718 Rat Anti-Mouse FoxP3

Description

Two-color flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. BALB/c mouse splenic leucocytes were stained with PE Rat anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 , and the cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No, 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse Foxp3 antibody Cat. No. 567095; Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. BALB/c mouse splenic leucocytes were stained with PE Rat anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 , and the cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No, 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse Foxp3 antibody Cat. No. 567095; Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. BALB/c mouse splenic leucocytes were stained with PE Rat anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 , and the cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No, 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse Foxp3 antibody Cat. No. 567095; Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. BALB/c mouse splenic leucocytes were stained with PE Rat anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 , and the cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No, 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse Foxp3 antibody Cat. No. 567095; Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. BALB/c mouse splenic leucocytes were stained with PE Rat anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 , and the cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No, 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse Foxp3 antibody Cat. No. 567095; Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. BALB/c mouse splenic leucocytes were stained with PE Rat anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 , and the cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Rat IgG2b, kappa Isotype Control Cat. No, 566943; Left Plot or BD Horizon R718 Rat Anti-Mouse Foxp3 antibody Cat. No. 567095; Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.