BD HorizonTM R718 Rat Anti-Mouse Ly-6C

Description

Two-color flow cytometric analysis of Ly-6C expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 503030/503031/561966 and either BD Horizon R718 Rat IgM, kappa Isotype Control Cat. No. 566950; Left Plot or BD Horizon R718 Rat Anti-Mouse Ly-6C Cat. No. 566987; Right Plot at 1 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 503030/503031/561966 and either BD Horizon R718 Rat IgM, kappa Isotype Control Cat. No. 566950; Left Plot or BD Horizon R718 Rat Anti-Mouse Ly-6C Cat. No. 566987; Right Plot at 1 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 503030/503031/561966 and either BD Horizon R718 Rat IgM, kappa Isotype Control Cat. No. 566950; Left Plot or BD Horizon R718 Rat Anti-Mouse Ly-6C Cat. No. 566987; Right Plot at 1 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 503030/503031/561966 and either BD Horizon R718 Rat IgM, kappa Isotype Control Cat. No. 566950; Left Plot or BD Horizon R718 Rat Anti-Mouse Ly-6C Cat. No. 566987; Right Plot at 1 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 503030/503031/561966 and either BD Horizon R718 Rat IgM, kappa Isotype Control Cat. No. 566950; Left Plot or BD Horizon R718 Rat Anti-Mouse Ly-6C Cat. No. 566987; Right Plot at 1 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 503030/503031/561966 and either BD Horizon R718 Rat IgM, kappa Isotype Control Cat. No. 566950; Left Plot or BD Horizon R718 Rat Anti-Mouse Ly-6C Cat. No. 566987; Right Plot at 1 mug/test. A bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.