BD HorizonTM RB545 Hamster Anti-Mouse CD11c (Integrin alphaX)

Description

Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon RB545 Hamster IgG2, lambda1 Isotype Control Cat. No. 570168; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569747/569775; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon RB545 Hamster IgG2, lambda1 Isotype Control Cat. No. 570168; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569747/569775; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon RB545 Hamster IgG2, lambda1 Isotype Control Cat. No. 570168; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569747/569775; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon RB545 Hamster IgG2, lambda1 Isotype Control Cat. No. 570168; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569747/569775; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon RB545 Hamster IgG2, lambda1 Isotype Control Cat. No. 570168; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569747/569775; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon RB545 Hamster IgG2, lambda1 Isotype Control Cat. No. 570168; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569747/569775; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific.