BD HorizonTM RB545 Hamster Anti-Mouse CD3e

Description

Multicolor flow cytometric analysis of CD3e expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093 antibodies and with either BD Horizon RB545 Hamster IgG1, kappa Isotype Control Cat. No. 570321; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD3e antibody Cat. No. 569725/569753; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093 antibodies and with either BD Horizon RB545 Hamster IgG1, kappa Isotype Control Cat. No. 570321; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD3e antibody Cat. No. 569725/569753; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093 antibodies and with either BD Horizon RB545 Hamster IgG1, kappa Isotype Control Cat. No. 570321; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD3e antibody Cat. No. 569725/569753; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3e expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093 antibodies and with either BD Horizon RB545 Hamster IgG1, kappa Isotype Control Cat. No. 570321; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD3e antibody Cat. No. 569725/569753; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3e expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093 antibodies and with either BD Horizon RB545 Hamster IgG1, kappa Isotype Control Cat. No. 570321; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD3e antibody Cat. No. 569725/569753; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and APC Rat Anti-Mouse CD8a Cat. No. 553035/561093 antibodies and with either BD Horizon RB545 Hamster IgG1, kappa Isotype Control Cat. No. 570321; Left Plot or BD Horizon RB545 Hamster Anti-Mouse CD3e antibody Cat. No. 569725/569753; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific.