BD HorizonTM RB545 Mouse Anti-Human CCR7 (CD197)

Description

Multiparameter flow cytometric analysis of CCR7 CD197 expression on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , BD Horizon RY586 Mouse Anti-Human CD8 Cat. No. 568114/568115 , APC Mouse Anti-Human CD45RA Cat. No. 550855/561884 antibodies, and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284 or BD Horizon RB545 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 569271/569272 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Plots 1 and 2: The pseudocolor density plots showing the correlated expression of CCR7 CD197 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Multiparameter flow cytometric analysis of CCR7 CD197 expression on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , BD Horizon RY586 Mouse Anti-Human CD8 Cat. No. 568114/568115 , APC Mouse Anti-Human CD45RA Cat. No. 550855/561884 antibodies, and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284 or BD Horizon RB545 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 569271/569272 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Plots 1 and 2: The pseudocolor density plots showing the correlated expression of CCR7 CD197 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Multiparameter flow cytometric analysis of CCR7 CD197 expression on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , BD Horizon RY586 Mouse Anti-Human CD8 Cat. No. 568114/568115 , APC Mouse Anti-Human CD45RA Cat. No. 550855/561884 antibodies, and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284 or BD Horizon RB545 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 569271/569272 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Plots 1 and 2: The pseudocolor density plots showing the correlated expression of CCR7 CD197 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes.