BD HorizonTM RB545 Mouse Anti-Human CD107a (LAMP-1)

Description

Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 569737/569765; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 569737/569765; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 569737/569765; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software.