BD HorizonTM RB545 Mouse Anti-Human CD16

Description

Multiparameter flow cytometric analysis of CD16 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 568219 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human CD16 antibody Cat. No. 569242/569243; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD16 or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A, Bottom Plots signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or intact leucocytes Bottom Plots . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multiparameter flow cytometric analysis of CD16 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 568219 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human CD16 antibody Cat. No. 569242/569243; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD16 or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A, Bottom Plots signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or intact leucocytes Bottom Plots . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Multiparameter flow cytometric analysis of CD16 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 568219 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human CD16 antibody Cat. No. 569242/569243; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD16 or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A, Bottom Plots signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or intact leucocytes Bottom Plots . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software.