BD HorizonTM RB545 Mouse Anti-Human CD20

Description

Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RB545 Mouse IgG2b, kappa Isotype Control Cat. No. 569292; Left Plot or BD Horizon RB545 Mouse Anti-Human CD20 antibody Cat. No. 569729/569757; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RB545 Mouse IgG2b, kappa Isotype Control Cat. No. 569292; Left Plot or BD Horizon RB545 Mouse Anti-Human CD20 antibody Cat. No. 569729/569757; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RB545 Mouse IgG2b, kappa Isotype Control Cat. No. 569292; Left Plot or BD Horizon RB545 Mouse Anti-Human CD20 antibody Cat. No. 569729/569757; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RB545 Mouse IgG2b, kappa Isotype Control Cat. No. 569292; Left Plot or BD Horizon RB545 Mouse Anti-Human CD20 antibody Cat. No. 569729/569757; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RB545 Mouse IgG2b, kappa Isotype Control Cat. No. 569292; Left Plot or BD Horizon RB545 Mouse Anti-Human CD20 antibody Cat. No. 569729/569757; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RB545 Mouse IgG2b, kappa Isotype Control Cat. No. 569292; Left Plot or BD Horizon RB545 Mouse Anti-Human CD20 antibody Cat. No. 569729/569757; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software.