BD HorizonTM RB545 Mouse Anti-Human CD24

Description

Multiparameter flow cytometric analysis of CD24 expression on Human peripheral blood leukocytes. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742; Top Plots and with either BD Horizon RB545 Mouse IgG2a, kappa Isotype Control Cat. No. 569277; Left Plots or BD Horizon RB545 Mouse Anti-Human CD24 antibody Cat. No. 569746/569774; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of CD24 or Ig Isotype control staining versus CD19 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Top Plots or leukocyte populations Bottom Plots . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multiparameter flow cytometric analysis of CD24 expression on Human peripheral blood leukocytes. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742; Top Plots and with either BD Horizon RB545 Mouse IgG2a, kappa Isotype Control Cat. No. 569277; Left Plots or BD Horizon RB545 Mouse Anti-Human CD24 antibody Cat. No. 569746/569774; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of CD24 or Ig Isotype control staining versus CD19 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Top Plots or leukocyte populations Bottom Plots . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Multiparameter flow cytometric analysis of CD24 expression on Human peripheral blood leukocytes. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742; Top Plots and with either BD Horizon RB545 Mouse IgG2a, kappa Isotype Control Cat. No. 569277; Left Plots or BD Horizon RB545 Mouse Anti-Human CD24 antibody Cat. No. 569746/569774; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of CD24 or Ig Isotype control staining versus CD19 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Top Plots or leukocyte populations Bottom Plots . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software.