BD HorizonTM RB545 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Multicolor flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and stimulated Human peripheral blood lymphocytes. Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569267/569268; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-parameter pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284, dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 569267/569268, solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multicolor flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and stimulated Human peripheral blood lymphocytes. Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569267/569268; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-parameter pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284, dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 569267/569268, solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multicolor flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and stimulated Human peripheral blood lymphocytes. Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569267/569268; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-parameter pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284, dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 569267/569268, solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Multicolor flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and stimulated Human peripheral blood lymphocytes. Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569267/569268; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-parameter pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284, dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 569267/569268, solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Multicolor flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and stimulated Human peripheral blood lymphocytes. Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569267/569268; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-parameter pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284, dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 569267/569268, solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multicolor flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and stimulated Human peripheral blood lymphocytes. Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841/555349/561840 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 569267/569268; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-parameter pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284, dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 569267/569268, solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software.