BD HorizonTM RB545 Mouse Anti-Human CD314 (NKG2D)

Description

Multiparameter flow cytometric analysis of CD314 NKG2D expression on Human peripheral blood leukocytes. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with APC Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555518 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 570932/571004; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multiparameter flow cytometric analysis of CD314 NKG2D expression on Human peripheral blood leukocytes. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with APC Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555518 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 570932/571004; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Multiparameter flow cytometric analysis of CD314 NKG2D expression on Human peripheral blood leukocytes. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with APC Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555518 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 570932/571004; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software.