BD HorizonTM RB545 Mouse Anti-Human CD45RO

Description

Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leucocyte populations . Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD HorizonBV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885; Top Plots and with either BD Horizon RB545 Mouse IgG2a, kappa Isotype Control Cat. No. 569277; Left Plots or BD Horizon RB545 Mouse Anti-Human CD45RO antibody Cat. No. 569750/569778; Right Plots . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Top Plots : The bivariate pseudocolor density plot showing the correlated expression CD45RO or Ig isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots : The bivariate pseudocolor density plot showing the correlated expression of CD45RO or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leucocyte populations . Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD HorizonBV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885; Top Plots and with either BD Horizon RB545 Mouse IgG2a, kappa Isotype Control Cat. No. 569277; Left Plots or BD Horizon RB545 Mouse Anti-Human CD45RO antibody Cat. No. 569750/569778; Right Plots . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Top Plots : The bivariate pseudocolor density plot showing the correlated expression CD45RO or Ig isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots : The bivariate pseudocolor density plot showing the correlated expression of CD45RO or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Show More Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leucocyte populations . Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD HorizonBV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885; Top Plots and with either BD Horizon RB545 Mouse IgG2a, kappa Isotype Control Cat. No. 569277; Left Plots or BD Horizon RB545 Mouse Anti-Human CD45RO antibody Cat. No. 569750/569778; Right Plots . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Top Plots : The bivariate pseudocolor density plot showing the correlated expression CD45RO or Ig isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots : The bivariate pseudocolor density plot showing the correlated expression of CD45RO or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations.