BD HorizonTM RB545 Mouse Anti-Human CD5

Description

Multiparameter flow cytometric analysis of CD5 expression on Human peripheral blood leucocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leucocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD5 antibody Cat. No. 569751/569779; Right Plot . The bivariate pseudocolor density plot showing CD5 expression or Ig Isotype control staining versus side light scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multiparameter flow cytometric analysis of CD5 expression on Human peripheral blood leucocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leucocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD5 antibody Cat. No. 569751/569779; Right Plot . The bivariate pseudocolor density plot showing CD5 expression or Ig Isotype control staining versus side light scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multiparameter flow cytometric analysis of CD5 expression on Human peripheral blood leucocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leucocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD5 antibody Cat. No. 569751/569779; Right Plot . The bivariate pseudocolor density plot showing CD5 expression or Ig Isotype control staining versus side light scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Multiparameter flow cytometric analysis of CD5 expression on Human peripheral blood leucocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leucocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD5 antibody Cat. No. 569751/569779; Right Plot . The bivariate pseudocolor density plot showing CD5 expression or Ig Isotype control staining versus side light scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Multiparameter flow cytometric analysis of CD5 expression on Human peripheral blood leucocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leucocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD5 antibody Cat. No. 569751/569779; Right Plot . The bivariate pseudocolor density plot showing CD5 expression or Ig Isotype control staining versus side light scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multiparameter flow cytometric analysis of CD5 expression on Human peripheral blood leucocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leucocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD5 antibody Cat. No. 569751/569779; Right Plot . The bivariate pseudocolor density plot showing CD5 expression or Ig Isotype control staining versus side light scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software.