BD HorizonTM RB545 Mouse Anti-Human CD56 (NCAM-1)

Description

Multiparameter flow cytometric analysis of CD56 NCAM-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD16 antibody Cat. No. 563785 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 569263/569264; Right Plots . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of CD56 NCAM-1 or Ig Isotype control staining versus CD16 Top Plots or side light-scatter SSC-A, Bottom Plots signals were derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes Top Plots or intact leucocytes Bottom Plots . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multiparameter flow cytometric analysis of CD56 NCAM-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD16 antibody Cat. No. 563785 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 569263/569264; Right Plots . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of CD56 NCAM-1 or Ig Isotype control staining versus CD16 Top Plots or side light-scatter SSC-A, Bottom Plots signals were derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes Top Plots or intact leucocytes Bottom Plots . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Multiparameter flow cytometric analysis of CD56 NCAM-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD16 antibody Cat. No. 563785 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 569263/569264; Right Plots . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of CD56 NCAM-1 or Ig Isotype control staining versus CD16 Top Plots or side light-scatter SSC-A, Bottom Plots signals were derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes Top Plots or intact leucocytes Bottom Plots . Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software.