BD HorizonTM RB545 Mouse Anti-Human CD86 (B7-2)

Description

Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569734/569762; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569734/569762; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569734/569762; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569734/569762; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569734/569762; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569734/569762; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software.