BD HorizonTM RB545 Mouse Anti-Human CD90 (Thy-1)

Description

Multicolor flow cytometric analysis of CD90 Thy-1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD34 antibody Cat. No. 555824/560940 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 569731/569759; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 Thy-1 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Flow cytometric analysis of CD90 Thy-1 expression on Human HEL cells. Cells from the HEL Human erythroleukemia, ATCC TIB-180 cell line were stained with BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 569731/569759; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 Thy-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multicolor flow cytometric analysis of CD90 Thy-1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD34 antibody Cat. No. 555824/560940 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 569731/569759; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 Thy-1 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Flow cytometric analysis of CD90 Thy-1 expression on Human HEL cells. Cells from the HEL Human erythroleukemia, ATCC TIB-180 cell line were stained with BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 569731/569759; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 Thy-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Multicolor flow cytometric analysis of CD90 Thy-1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD34 antibody Cat. No. 555824/560940 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 569731/569759; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 Thy-1 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Flow cytometric analysis of CD90 Thy-1 expression on Human HEL cells. Cells from the HEL Human erythroleukemia, ATCC TIB-180 cell line were stained with BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; dashed line histogram or BD Horizon RB545 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 569731/569759; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 Thy-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software.