BD HorizonTM RB545 Mouse Anti-Human Granzyme B

Description

Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369/561421/561952 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human Granzyme B antibody Cat. No. 569290/569291; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369/561421/561952 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human Granzyme B antibody Cat. No. 569290/569291; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369/561421/561952 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human Granzyme B antibody Cat. No. 569290/569291; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369/561421/561952 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human Granzyme B antibody Cat. No. 569290/569291; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369/561421/561952 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human Granzyme B antibody Cat. No. 569290/569291; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369/561421/561952 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human Granzyme B antibody Cat. No. 569290/569291; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software.