BD HorizonTM RB545 Mouse Anti-Human TCRalphabeta

Description

Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD19 antibody Cat. No. 563549 , BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human TCRalphabeta antibody Cat. No. 569741/569769; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 Upper Plots or CD3 Lower Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD19 antibody Cat. No. 563549 , BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human TCRalphabeta antibody Cat. No. 569741/569769; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 Upper Plots or CD3 Lower Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Show More Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD19 antibody Cat. No. 563549 , BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Human TCRalphabeta antibody Cat. No. 569741/569769; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 Upper Plots or CD3 Lower Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software.