BD HorizonTM RB545 Mouse Anti-Ki-67

Description

Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the Human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RB545 Mouse IgG1 Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Ki-67 antibody Cat. No. 569275/569276; Right Plots and counterstained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DAPI staining versus Ki-67 or Ig isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the Human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RB545 Mouse IgG1 Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Ki-67 antibody Cat. No. 569275/569276; Right Plots and counterstained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DAPI staining versus Ki-67 or Ig isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the Human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RB545 Mouse IgG1 Isotype Control Cat. No. 569284; Left Plots or BD Horizon RB545 Mouse Anti-Ki-67 antibody Cat. No. 569275/569276; Right Plots and counterstained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DAPI staining versus Ki-67 or Ig isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software.