BD HorizonTM RB545 Mouse Anti-T-bet

Description

Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-T-bet antibody Cat. No. 569730/569758; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Data shown on this Technical Data Sheet are not lot specific . Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-T-bet antibody Cat. No. 569730/569758; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Data shown on this Technical Data Sheet are not lot specific . Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-T-bet antibody Cat. No. 569730/569758; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Data shown on this Technical Data Sheet are not lot specific . Show More Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-T-bet antibody Cat. No. 569730/569758; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Data shown on this Technical Data Sheet are not lot specific . Show More Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-T-bet antibody Cat. No. 569730/569758; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Data shown on this Technical Data Sheet are not lot specific . Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-T-bet antibody Cat. No. 569730/569758; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Data shown on this Technical Data Sheet are not lot specific .