BD HorizonTM RB545 Rat Anti-Mouse I-A/I-E

Description

Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB545 Rat IgG2b, kappa Isotype Control Cat. No. 569286; Left Plot or BD Horizon RB545 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569744/569772; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB545 Rat IgG2b, kappa Isotype Control Cat. No. 569286; Left Plot or BD Horizon RB545 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569744/569772; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB545 Rat IgG2b, kappa Isotype Control Cat. No. 569286; Left Plot or BD Horizon RB545 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569744/569772; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB545 Rat IgG2b, kappa Isotype Control Cat. No. 569286; Left Plot or BD Horizon RB545 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569744/569772; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB545 Rat IgG2b, kappa Isotype Control Cat. No. 569286; Left Plot or BD Horizon RB545 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569744/569772; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB545 Rat IgG2b, kappa Isotype Control Cat. No. 569286; Left Plot or BD Horizon RB545 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569744/569772; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific.