BD HorizonTM RB545 Rat Anti-Mouse Ly-6G

Description

Flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB545 Rat IgG2a, kappa Isotype Control Cat. No. 568130; dashed line histogram or BD Horizon RB545 Rat Anti-Mouse Ly-6G antibody Cat. No. 569726/569754; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Ly-6G or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were stained with either BD Horizon RB545 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RB545 Rat Anti-Mouse Ly-6G antibody Cat. No. 569726/569754; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6G or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB545 Rat IgG2a, kappa Isotype Control Cat. No. 568130; dashed line histogram or BD Horizon RB545 Rat Anti-Mouse Ly-6G antibody Cat. No. 569726/569754; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Ly-6G or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were stained with either BD Horizon RB545 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RB545 Rat Anti-Mouse Ly-6G antibody Cat. No. 569726/569754; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6G or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB545 Rat IgG2a, kappa Isotype Control Cat. No. 568130; dashed line histogram or BD Horizon RB545 Rat Anti-Mouse Ly-6G antibody Cat. No. 569726/569754; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Ly-6G or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow cells. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Ly-6G expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were stained with either BD Horizon RB545 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RB545 Rat Anti-Mouse Ly-6G antibody Cat. No. 569726/569754; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6G or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 software. Data shown on this Technical Data Sheet are not lot specific.