BD HorizonTM RB613 Mouse Anti-Human CD152 (CTLA-4)

Description

Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG2a, kappa Isotype Control Cat. No. 571196; Left Plot or BD Horizon RB613 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 571254/571314; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG2a, kappa Isotype Control Cat. No. 571196; Left Plot or BD Horizon RB613 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 571254/571314; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG2a, kappa Isotype Control Cat. No. 571196; Left Plot or BD Horizon RB613 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 571254/571314; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG2a, kappa Isotype Control Cat. No. 571196; Left Plot or BD Horizon RB613 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 571254/571314; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG2a, kappa Isotype Control Cat. No. 571196; Left Plot or BD Horizon RB613 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 571254/571314; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG2a, kappa Isotype Control Cat. No. 571196; Left Plot or BD Horizon RB613 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 571254/571314; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.