BD HorizonTM RB613 Mouse Anti-Human CD197 (CCR7)

Description

Multicolor flow cytometric analysis of CD197 CCR7 on Human peripheral blood leukocytes and lymphocytes Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795 , APC Mouse Anti-Human CD45RA Cat. No. 550855 antibodies, and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106 or BD Horizon RB613 Mouse Anti-Human CD197 CCR7 antibody Cat. No. 571209/571213 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Plots 1 and 2: The bivariate pseudocolor density plots showing the correlated expression of CD197 CCR7 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CD197 CCR7 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CD197 CCR7 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CD197 CCR7 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Multicolor flow cytometric analysis of CD197 CCR7 on Human peripheral blood leukocytes and lymphocytes Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795 , APC Mouse Anti-Human CD45RA Cat. No. 550855 antibodies, and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106 or BD Horizon RB613 Mouse Anti-Human CD197 CCR7 antibody Cat. No. 571209/571213 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Plots 1 and 2: The bivariate pseudocolor density plots showing the correlated expression of CD197 CCR7 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CD197 CCR7 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CD197 CCR7 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CD197 CCR7 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Show More Multicolor flow cytometric analysis of CD197 CCR7 on Human peripheral blood leukocytes and lymphocytes Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795 , APC Mouse Anti-Human CD45RA Cat. No. 550855 antibodies, and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106 or BD Horizon RB613 Mouse Anti-Human CD197 CCR7 antibody Cat. No. 571209/571213 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Plots 1 and 2: The bivariate pseudocolor density plots showing the correlated expression of CD197 CCR7 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CD197 CCR7 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CD197 CCR7 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CD197 CCR7 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes.