BD HorizonTM RB613 Mouse Anti-Human CD20

Description

Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plot or BD Horizon RB613 Mouse Anti-Human CD20 antibody Cat. No. 571119/571120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plot or BD Horizon RB613 Mouse Anti-Human CD20 antibody Cat. No. 571119/571120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plot or BD Horizon RB613 Mouse Anti-Human CD20 antibody Cat. No. 571119/571120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plot or BD Horizon RB613 Mouse Anti-Human CD20 antibody Cat. No. 571119/571120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plot or BD Horizon RB613 Mouse Anti-Human CD20 antibody Cat. No. 571119/571120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were stained with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plot or BD Horizon RB613 Mouse Anti-Human CD20 antibody Cat. No. 571119/571120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.