BD HorizonTM RB613 Mouse Anti-Human CD45RA

Description

Multiparameter flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with APC Mouse Anti-Human CD45RO antibody Cat. No. 559865 and with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plots or BD Horizon RB613 Mouse Anti-Human CD45RA antibody Cat. No. 571256/571316; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of either CD45RA or Ig Isotype control staining versus CD45RO Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with APC Mouse Anti-Human CD45RO antibody Cat. No. 559865 and with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plots or BD Horizon RB613 Mouse Anti-Human CD45RA antibody Cat. No. 571256/571316; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of either CD45RA or Ig Isotype control staining versus CD45RO Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with APC Mouse Anti-Human CD45RO antibody Cat. No. 559865 and with either BD Horizon RB613 Mouse IgG2b, kappa Isotype Control Cat. No. 571121; Left Plots or BD Horizon RB613 Mouse Anti-Human CD45RA antibody Cat. No. 571256/571316; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of either CD45RA or Ig Isotype control staining versus CD45RO Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.