BD HorizonTM RB613 Mouse Anti-Human KLRG1

Description

Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plots or BD Horizon RB613 Mouse Anti-Human KLRG1 antibody Cat. No. 571358/571360; Right Plots . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plots or BD Horizon RB613 Mouse Anti-Human KLRG1 antibody Cat. No. 571358/571360; Right Plots . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plots or BD Horizon RB613 Mouse Anti-Human KLRG1 antibody Cat. No. 571358/571360; Right Plots . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.