BD HorizonTM RB613 Mouse Anti-T-bet

Description

Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-T-bet antibody Cat. No. 571286/571346; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-T-bet antibody Cat. No. 571286/571346; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-T-bet antibody Cat. No. 571286/571346; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-T-bet antibody Cat. No. 571286/571346; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-T-bet antibody Cat. No. 571286/571346; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-T-bet antibody Cat. No. 571286/571346; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.