BD HorizonTM RB613 Mouse Anti-TCF7 (TCF1)

Description

Two-color flow cytometric analysis of TCF7 TCF1 expression by Human lymphocytes or Mouse splenic leukocytes. Top Plots - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The cells were then stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-TCF7 TCF1 antibody Cat. No. 571348/571352; Right Plot at 0.5 mug/test. Bottom Plots - Mouse splenic leukocytes were similarly fixed and permeabilized. The cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB613 Mouse IgG1, kappa. Isotype Control Left Plot or BD Horizon RB613 Mouse Anti-TCF7 TCF1 antibody Right Plot at 0.5 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD4 versus TCF7 TCF1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact Human lymphocytes Top Plots or Mouse splenic leukocytes Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCF7 TCF1 expression by Human lymphocytes or Mouse splenic leukocytes. Top Plots - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The cells were then stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-TCF7 TCF1 antibody Cat. No. 571348/571352; Right Plot at 0.5 mug/test. Bottom Plots - Mouse splenic leukocytes were similarly fixed and permeabilized. The cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB613 Mouse IgG1, kappa. Isotype Control Left Plot or BD Horizon RB613 Mouse Anti-TCF7 TCF1 antibody Right Plot at 0.5 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD4 versus TCF7 TCF1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact Human lymphocytes Top Plots or Mouse splenic leukocytes Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCF7 TCF1 expression by Human lymphocytes or Mouse splenic leukocytes. Top Plots - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The cells were then stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plot or BD Horizon RB613 Mouse Anti-TCF7 TCF1 antibody Cat. No. 571348/571352; Right Plot at 0.5 mug/test. Bottom Plots - Mouse splenic leukocytes were similarly fixed and permeabilized. The cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB613 Mouse IgG1, kappa. Isotype Control Left Plot or BD Horizon RB613 Mouse Anti-TCF7 TCF1 antibody Right Plot at 0.5 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD4 versus TCF7 TCF1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact Human lymphocytes Top Plots or Mouse splenic leukocytes Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.