BD HorizonTM RB613 Rat Anti-Mouse CD19

Description

Multicolor flow cytometric analysis of CD19 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB613 Rat IgG2a, kappa Isotype Control Cat. No. 571107; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD19 antibody Cat. No. 571233/571297; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated expression patterns of CD19 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD19 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB613 Rat IgG2a, kappa Isotype Control Cat. No. 571107; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD19 antibody Cat. No. 571233/571297; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated expression patterns of CD19 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD19 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB613 Rat IgG2a, kappa Isotype Control Cat. No. 571107; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD19 antibody Cat. No. 571233/571297; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated expression patterns of CD19 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD19 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB613 Rat IgG2a, kappa Isotype Control Cat. No. 571107; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD19 antibody Cat. No. 571233/571297; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated expression patterns of CD19 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD19 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB613 Rat IgG2a, kappa Isotype Control Cat. No. 571107; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD19 antibody Cat. No. 571233/571297; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated expression patterns of CD19 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD19 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB613 Rat IgG2a, kappa Isotype Control Cat. No. 571107; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD19 antibody Cat. No. 571233/571297; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated expression patterns of CD19 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.