BD HorizonTM RB613 Rat Anti-Mouse CD4

Description

Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RB613 Rat IgG2b, kappa Isotype Control Cat. No. 571108; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD4 antibody Cat. No. 571101/571102; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RB613 Rat IgG2b, kappa Isotype Control Cat. No. 571108; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD4 antibody Cat. No. 571101/571102; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RB613 Rat IgG2b, kappa Isotype Control Cat. No. 571108; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD4 antibody Cat. No. 571101/571102; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RB613 Rat IgG2b, kappa Isotype Control Cat. No. 571108; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD4 antibody Cat. No. 571101/571102; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RB613 Rat IgG2b, kappa Isotype Control Cat. No. 571108; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD4 antibody Cat. No. 571101/571102; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RB613 Rat IgG2b, kappa Isotype Control Cat. No. 571108; Left Plot or BD Horizon RB613 Rat Anti-Mouse CD4 antibody Cat. No. 571101/571102; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.